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Image Search Results
Journal: American Journal of Translational Research
Article Title: Inhibition of SIRT1 promotes taste bud stem cell survival and mitigates radiation-induced oral mucositis in mice
doi:
Figure Lengend Snippet: SIRT1 inhibition stimulates the regeneration of taste bud organoid after irradiation. A. Bright field images of taste bud organoids treated with or without NAM at day 10 after IR. B. Representative images of taste bud organoid stained with DAPI and EdU; Scale bar 100 μm. C. Statistical analysis of the organoid size (surface area in pixels). D. Percentage of EdU positive area in taste bud organoid treated with or without NAM after IR. Data presented as the mean ± SD, n=55 from three independent tests, ***P<0.001.
Article Snippet: SIRT1 inhibitors: 10 mM
Techniques: Inhibition, Irradiation, Staining
Journal: American Journal of Translational Research
Article Title: Inhibition of SIRT1 promotes taste bud stem cell survival and mitigates radiation-induced oral mucositis in mice
doi:
Figure Lengend Snippet: NAM suppresses the histological signs of oral mucositis induced by single dose irradiation. A. Single dose radiation and drug treatment scheme for mice. A single dose of Nicotinamide (1000 mg/kg, NAM) or PBS administered 24 h after 15 Gy IR. B. Representative pictures of tongues stained with TB and its histological analysis (right panels, HE stained), from control mice (non-irradiated, NR), and irradiated mice on day 10 post IR, after they received vehicle (PBS) or NAM. Scale bar 200 μm. C. Mean ulcer size percentage to the total epithelialized upper surface of the tongue. D. Quantification of the posterior epithelium height. Data presented as the mean ± SD (n=8), *P<0.05, ***P<0.001.
Article Snippet: SIRT1 inhibitors: 10 mM
Techniques: Irradiation, Staining
Journal: American Journal of Translational Research
Article Title: Inhibition of SIRT1 promotes taste bud stem cell survival and mitigates radiation-induced oral mucositis in mice
doi:
Figure Lengend Snippet: SIRT1 inhibitors promote the survival of Lgr5+ taste bud stem cells in mice. A. Radiation and drug treatment scheme for mice. Tissue collected on day 3, 5, 7 and 9 from groups with or without NAM injection. B. Representative images of stem cells after IR, Scale bar 100 μm. C. Quantitative comparison of Lgr5+ taste bud stem cell depletion kinetics after 15 Gy IR in CV papilla tissue. Data presented as the mean ± SD (n=4). *P<0.05, ***P<0.001.
Article Snippet: SIRT1 inhibitors: 10 mM
Techniques: Injection
Journal: American Journal of Translational Research
Article Title: Inhibition of SIRT1 promotes taste bud stem cell survival and mitigates radiation-induced oral mucositis in mice
doi:
Figure Lengend Snippet: Sirt1 inhibitors mitigate radiation toxicity in taste bud organoid. A. Representative images of taste bud organoid treated with vehicle or SIRT1 inhibitors (NAM, EX527, salermide and sirtinol) or SIRT2 inhibitors (AGK2 and sirReal2) at day 10 after 8 Gy IR, Scale bar 100 μm. B. Statistical analysis of the average sizes (surface area in pixels, mean ± SD) of taste bud organoids after 8 Gy IR. C. Distribution of organoids by size after 8 Gy IR. D. Statistical analysis of the average sizes (surface area in pixels, mean ± SD) of taste bud organoids after 6 Gy IR. E. Distribution of organoids by size after 6 Gy IR. n=68 from three independent tests. *P<0.05, **P<0.01, ***P<0.001 vs. Ctrl (PBS), NS indicates no significance.
Article Snippet: SIRT1 inhibitors: 10 mM
Techniques: